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Index > Protein center > Aadat(Gene name) > Mouse
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  • Aadat (Gene name),
  • Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial (Protein name ),  AADAT_MOUSE from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    Aadat(Kat2);
    Protein name:
    Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial(KAT/AadAT);
    Alternative:
    2.6.1.39;2-aminoadipate transaminase);2-aminoadipate aminotransferase;KAT/AadAT;Alpha-aminoadipate aminotransferase(AadAT);2.6.1.7;Kynurenine--oxoglutarate aminotransferase II);Kynurenine aminotransferase II;Kynurenine--oxoglutarate transaminase II;
    Organism:
    Mouse (Mus musculus). 
    General Annotation
    Sub Unit:
    Homodimer.
    Function:
    Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro).
    Subcellular Location:
    Mitochondrion
    Protein Attributes:
    Sequence length:
    425
    Sequence:
    50:
    MNYSRFLTAT | SLARKPSPIR | TTADILSKAP | KTLISLAPGS | PNPSMFPFKS | 
    100:
    AAFTVENGST | IRFEDDLIKR | ALQYSPSYGI | PELLSWLKQF | QVKLHNPPTV | 
    150:
    NYPPNQGQMD | LCITSGCQDG | LCKAFEMLIN | PGDTILVNEP | LFPGTLYAMK | 
    200:
    PLGCNIINVP | SDEHGIIPEG | LKKILSQWKP | EDSKDPTKKT | PKFLYTVPNG | 
    250:
    NNPTGNSLTG | DRKKEIYELA | RKYDFLIIED | DPYYFLQFSK | PWEPTFLSMD | 
    300:
    VDGRVIRADT | FSKTVSSGLR | VGFMTGPKTL | IQNIVLHTQV | SSVHACTLSQ | 
    350:
    LMILQLLHQW | GEEGFLAHID | RTIDFYKNQR | DSILAAADKW | LRGLAEWHVP | 
    400:
    KAGMFLWIKV | KGISDTKQLI | EEKAIEREVL | LVPGNGFFID | GSAPTSFFRA | 
    425:
    SFSLATPAQM | DTAFQRLAQL | IKESL
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    SMR:
    UniGene:
    String:
    Pfam:
    KEGG:
    Uniprot:
     
    FOR
    ELISA Kit for Mouse KAT/AadAT
    MSDS:
    Please sign in first.
    ELISA Kit for Mouse KAT/AadAT
    MSDS:
    Please sign in first.
    ELISA Kit for Mouse KAT/AadAT
    Cat.:
    E12537r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Mouse KAT/AadAT
    Cat.:
    E12537m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse KAT/AadAT
    Cat.:
    U12537h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse KAT/AadAT
    Cat.:
    U12537r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Mouse KAT/AadAT
    MSDS:
    Please sign in first.
    CLIA Kit for Mouse KAT/AadAT
    Cat.:
    U12537m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Mouse KAT/AadAT
    Cat.:
    P12537Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse KAT/AadAT
    Polyclonal Antibody for Mouse KAT/AadAT
    Polyclonal Antibody for Mouse KAT/AadAT
    Monoclonal Antibody for Mouse KAT/AadAT
    Monoclonal Antibody for Mouse KAT/AadAT
    Monoclonal Antibody for Mouse KAT/AadAT
    Monoclonal Antibody for Mouse KAT/AadAT
    Protein for Mouse KAT/AadAT
    Protein for Mouse KAT/AadAT
    Protein for Mouse KAT/AadAT
    Protein for Mouse KAT/AadAT

    R&D Technical Data
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    s

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    s
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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Precision
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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤5.7%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤8.2%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Recovery
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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Recovery was determined by spiking various levels of AADAT into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    97

    94-103

    Plasma

    98

    93-105

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Linearity
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AADAT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    93-101%

    91-104%

    95-100%

    92-101%

    EDTA plasma(n=5)

    90-105%

    95-103%

    90-108%

    94-102%

    heparin plasma(n=5)

    92-103%

     

    93-102%

    92-105%

    91-106%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    References
    1. 1.
      "Genomic organization and expression analysis of mouse kynurenine aminotransferase II, a possible factor in the pathophysiology of Huntington's disease."
      Yu P. , Mosbrook D.M. , Tagle D.A.
      Mamm. Genome10:845-852(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];TISSUE SPECIFICITY
      strain: 129/SvJ.
    2. 2.
      "The transcriptional landscape of the mammalian genome."
      Carninci P. , Kasukawa T. , Katayama S. , Gough J. , Frith M.C. , Maeda N. , Oyama R. , Ravasi T. , Lenhard B. , Wells C. , Kodzius R. , Shimokawa K. , Bajic V.B. , Brenner S.E. , Batalov S. , Forrest A.R. , Zavolan M. , Davis M.J. , more...
      Science309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      strain: C57BL/6J.
      tissue: Kidney.
    3. 3.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Liver.
    4. 4.
      "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J. , Chen Y. , Tishkoff D.X. , Peng C. , Tan M. , Dai L. , Xie Z. , Zhang Y. , Zwaans B.M. , Skinner M.E. , Lombard D.B. , Zhao Y.
      Mol. Cell50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-172; LYS-263; LYS-339 AND LYS-367;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Liver.
    5. 5.
      "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
      Rardin M.J. , Newman J.C. , Held J.M. , Cusack M.P. , Sorensen D.J. , Li B. , Schilling B. , Mooney S.D. , Kahn C.R. , Verdin E. , Gibson B.W.
      Proc. Natl. Acad. Sci. U.S.A.110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-69; LYS-179; LYS-263; LYS-339; LYS-351; LYS-367 AND LYS-422;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Liver.
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